mRNAProduction
We use optimized processes to produce highly pure, stable RNA that is efficiently translated, whilst minimizing production of double stranded RNA (dsRNA). Construct design starts with a high quality plasmid DNA construct. We will guide you through the process of selection of 5’ and 3’ UTRs, poly(A) tail length and codon optimization.
Design and cloning upon request
- Vector optimization
- Vector with optimized polyA tail
- Sequencing
Plasmid production
- Host cell transformation
- RE digestion
- Sequencing
- Master cell bank available upon request
- Flexible scale
- Segregated suite for GMP-like plasmid production
pDNA linearization
- pDNA enzymatic digestion
- QC assay by AGE
IVT reaction
- Uncapped mRNA
- Post-Transcriptional capping
- Co-Transcriptional capping
- Optimized protocol to increase yield
- Pseudouridine modification upon request
Chromatography
- mRNA capture
- Choose your capture mode according to your project needs
- Precipitation protocol can be used according to your project needs
Formulation
- Concentration and buffer exchange (continuous or discontinuous UF/DF depending on the scale)
- Final sterile filtration
- Aliquots storage
APPLICATIONS
Takis delivers premium mRNA production solutions tailored to diverse applications, ensuring reliable solutions and driving innovation in science and medicine.
Basic research
Formulation discovery
mRNA preclinical vaccine
Gene therapy research
Preclinical studies
PRODUCT HIGHLIGHTS
Highest purity standard and process free from animal derived components. Our mRNA Production is optimized to ensure quality guaranteeing purity, efficiency, and reliability.