Mouse MonoclonalAntibodies
Takis’ custom monoclonal antibody platform utilizes DNA electroporation to immunize animals and hybridoma technology to generate the desired mouse monoclonal antibodies
Sequence Analysis, Antigen Design and Codon Optimization
cDNA Cloning and Confirmation of functional In vitro Expression
Sera analysis by FACS or ELISA
Somatic Fusion and Hybridoma Production
Screening of positive Hybridomas
Subcloning and Monoclonal Antibody Production
- There is no need for antigen synthesis or expression.
- Appropriate molecular engineering techniques are applied, including codon optimization, the use of a secretory leader sequence, and fusion with immunoenhancing moieties.
- The antigen is expressed endogenously by muscle and antigen-presenting cells, leading to the induction of conformational antibodies.
- The combination with immunomodulators further increases the probability of success.
- Induction of conformational antibodies
Monoclonal Antibodies Workflow
