The Mannan Induced Psoriatic Arthritis (MiP)
The mannan (Saccharomyces Cerevisiae) induced PsA model (MiP) in mice is a newly developed model in which both skin lesions resembling those seen in Ps as well as the joint inflammation of PsA can be found. Disease is mediated by TNF-a produced by macrophages which leads to T cell activation and IL-17A production. The increased level of IL-17A recruit neutrophils to joint and skin and results in an inflammatory response. Repeated mannan injections (every 7th day) give rise to a chronic relapsing disease.
Onset of joint and skin inflammation is seen within a few days after the mannan injection and slowly heals out after one week if not reactivated by a second mannan injection.
STUDY ENDPOINT
- Histopathologic analysis
- Macroscopic scores (skin and arthritis scores)
- Ear epidermal thickness
- Blood collections and analysis
- Stool collections (NGS analysis of microbiota)
- Tissue collections
- Cytokine analysis and other inflammatory markers (IL-6, IL-17a, TNFα, TGFβ)
VALIDATION DATA
