TNBS-Induced Colitis Model
Haptinizing agent 2,4,6-trinitrobenzene sulfonic acid (TNBS) dissolved in ethanol and intrarectally administered to a mouse through a special catheter is a suitable method to induce colitis. Ethanol is required to break the mucosal barrier, whereas TNBS is believed to haptenize colonic autologous or microbiota proteins rendering them immunogenic to the host immune system. CD4+ T cells are suggested to play a key role in inducing colitis in this mouse model. The acute TNBS colitis model is usually induced by a single intrarectal administration and then observed for 3 days.
STUDY ENDPOINT
- MPO levels
- DAI and Macroscopic scores
- Cytokine analysis and other inflammatory markers (TNFa, IL-1b, SAA, KC)
- Blood collections and analysis (Fe2+ and Hemoglobin)
- Stool collections (NGS analysis of microbiota)
- Tissue collections
- Intestinal permeability (FITC-Dextran)
- Histopathologic analysis
VALIDATION DATA
